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In some echinoderms, including starfish and sea urchinsa major portion of the exposed acrosomal content contains a protein that temporarily holds the sperm on the egg's surface.

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The aforementioned process describes the physiologically relevant events.

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After gating the cell population according to forward- and side-scatter the resulting data can be analysed E.

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The zona pellucida also releases Ca granules to prevent additional sperm from binding.

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Furthermore, chemical agents which bring about the reaction in vitro, such as the ionophores ionomycin or A, have been used to shed light on its regulatory mechanisms.

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The aforementioned process describes the physiologically relevant events.

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It is then expressed as a percentage of the counted cells.

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Part of the sperm's cell membrane then fuses with the egg cell's membrane, and the contents of the head sink into the egg.

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At least cells are viewed in an arbitrary fashion and classified as either acrosome intact fluorescing bright green or acrosome reacted no probe present, or only on the equatorial region.

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It is then expressed as a percentage of the counted cells.

Upon penetration, if all is occurring normally, the process of egg-activation occurs and the oocyte is said to have become activated.

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